Yes
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin B1 antibody. Counter stained with hematoxylin.
All lanes: Cyclin B1 Rabbit mAb at 1/1k dilutionLane 1 : Mouse liver lysates Lane 2 : Hela whole cell lysates Lane 3 : JK whole cell lysates Lane 4 : MCF-7 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 48 kDa Observed band size: 55 kDaExposure time: 4 seconds
All lanes :Cyclin B1 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HT-1080 cell lysateLane 2 : Cyclin B1 knockdown HT-1080 cell lysateLysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence Cyclin B1 antibody (48818) ICC/IF staining of Cyclin B1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48818 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
