产品详情
产品名称YAP1 Rabbit mAb
克隆号SU33-06
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名65 kDa Yes associated protein antibody
65 kDa Yes-associated protein antibody
COB1 antibody
YAp 1 antibody
YAP 65 antibody
YAP antibody
YAP1 antibody
YAP1_HUMAN antibody
YAP2 antibody
YAP65 antibody
yes -associated protein delta antibody
Yes associated protein 1 65kDa antibody
Yes associated protein 1 antibody
Yes associated protein 2 antibody
yes associated protein beta antibody
YKI antibody
Yorkie homolog antibody
数据库入口号Swiss-Prot#:P46937
Uniprot
P46937
计算分子量65 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of YAP1 on HepG2 cell lysates using anti-YAP1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-YAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-YAP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-YAP1 antibody. Counter stained with hematoxylin.
ICC staining YAP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining YAP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH/3T3 cells with YAP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Commitment to cell division occurs at a point late in the G1 phase of the cell cycle, termed Start. Passage through Start requires the activation of the Cdc28 protein kinase by the cell cycle-regulated G1 cyclins. Maximal expression of these G1 cyclins is induced by the heterodimeric transcription factor complex composed of Swi4 (also designated Art1) and Swi6. Swi4 is the DNA-binding subunit of this complex. In addition to binding Swi4, Swi6 forms a complex with Mbp1. This complex activates S-phase cyclins and genes involved in DNA synthesis Rpb1 is the largest subunit of the yeast RNA polymerase II. Srb4 is a basal transcription factor that is essential for the establishment of the transcription initiation apparatus. Stress factors induce transcription through the induction of various transcription factors. Yap1 activates expression in response to oxidative stress, while Msn2 and Msn4 mediate transcription via the stress response element (STRE).
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Yes
