产品详情
产品名称p16INK4A Rabbit mAb
克隆号SU0702
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CCM2 antibody CDK4 inhibitor p16 INK4 antibody CDK4I antibody CDKN2 antibody CDKN2A antibody Cell cycle negative regulator beta antibody CMM2 antibody Cyclin dependent kinase 4 inhibitor A antibody Cyclin dependent kinase inhibitor 2A (melanoma p16 inhibits CDK4) antibody Cyclin Dependent Kinase Inhibitor 2A antibody Cyclin dependent kinase inhibitor 2A isoform 4 antibody Cyclin dependent kinase inhibitor 2A isoforms 1/2/3 antibody Cyclin dependent kinase inhibitor p16 antibody INK4 antibody INK4A antibody MLM antibody MTS1 antibody Multiple tumor suppressor 1 antibody p14 antibody p16 antibody P16INK4 antibody p16INK4a antibody p19 antibody p19Arf antibody TP16 antibody
数据库入口号Swiss-Prot#:P42771
Uniprot
P42771
计算分子量17 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.
ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-p16 INK4A antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates, promote orderly progression through the cell cycle. The protein p16INK4A, identified as a negative regulator of the cell cycle, has been shown to bind to and inhibit the activity of the Cdk4/cyclin D complex. p19 ARF, which is unrelated to p16, arises from transcription of an alternative reading frame of the p16 gene. Like p16, p19 ARF has been shown to induce cell cycle arrest. Mice lacking p19 ARF but expressing functional p16 have been shown to develop tumors early in life. Further studies have indicated that p19 ARF may be disrupted in a large percentage of human T cell acute lymphoblastic leukemias.
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