产品详情
产品名称Androgen receptor Rabbit mAb
克隆号ST0453
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名AIS antibody ANDR_HUMAN antibody Androgen nuclear receptor variant 2 antibody Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) antibody Androgen receptor antibody androgen receptor splice variant 4b antibody AR antibody AR8 antibody DHTR antibody Dihydro testosterone receptor antibody Dihydrotestosterone receptor (DHTR) antibody Dihydrotestosterone receptor antibody HUMARA antibody HYSP1 antibody KD antibody Kennedy disease (KD) antibody NR3C4 antibody Nuclear receptor subfamily 3 group C member 4 (NR3C4) antibody Nuclear receptor subfamily 3 group C member 4 antibody SBMA antibody SMAX1 antibody Spinal and bulbar muscular atrophy (SBMA) antibody Spinal and bulbar muscular atrophy antibody Testicular Feminization (TFM) antibody TFM antibody
数据库入口号Swiss-Prot#:P10275
Uniprot
P10275
计算分子量99 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1000
IHC: 1:50-1:100
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Androgen receptor antibody. Counter stained with hematoxylin.
ICC staining Androgen receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Androgen receptor in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Androgen receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with Androgen receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Androgens exhibit a wide range of effects on the development, maintenance and regulation of male phenotype and male reproductive physiology. The androgen receptor (AR) is a member of the steroid superfamily of ligand-dependent transcription factors. ARs bind the two biologically active androgens, testosterone (T) and dihydrotestosterone (DHT), with high and nearly identical affinities; however, the rates of association and dissociation of T are about three times more rapid than those of DHT. This difference has resulted in speculation as to whether these differences in binding kinetics could account for the different physiological effects of T and DHT. A striking feature of AR is its rapid degradation in the absence of ligand. It is now well established that androgen binding results in an at least six-fold increase in androgen stability and that ligand-induced stabilization of AR is highly androgen- specific.
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