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MAP1LC3A Rabbit mAb #48865

是否有货: Yes
产品详情

产品名称MAP1LC3A Rabbit mAb

克隆号ST47-03

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP, FC

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名ATG8E antibody
Autophagy-related protein LC3 A antibody
Autophagy-related ubiquitin-like modifier LC3 A antibody
LC3 antibody
LC3A antibody
MAP1 light chain 3 like protein 1 antibody
MAP1 light chain 3-like protein 1 antibody
MAP1A/1B light chain 3 A antibody
MAP1A/MAP1B LC3 A antibody
MAP1A/MAP1B light chain 3 A antibody
MAP1ALC3 antibody
MAP1BLC3 antibody
Map1lc3a antibody
Microtubule associated proteins 1A/1B light chain 3 antibody
Microtubule-associated protein 1 light chain 3 alpha antibody
Microtubule-associated proteins 1A and 1B, light chain 3 antibody
Microtubule-associated proteins 1A/1B light chain 3A antibody
MLP3A_HUMAN antibody

数据库入口号Swiss-Prot#:Q9H492

Uniprot Q9H492

计算分子量14 kDa

实际分子量16 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MAP1LC3A in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MAP1LC3A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: MAP1LC3A Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : U-87 MG whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 7 seconds
All lanes: MAP1LC3A Rabbit mAb at 1/1k dilution
Lane 1 : Mouse brain lysates
Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 6 seconds
All lanes:MAP1LC3A Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : MAP1LC3A knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubule-associated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance micro-tubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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