产品详情
产品名称MAP1LC3A Rabbit mAb
克隆号ST47-03
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名ATG8E antibody
Autophagy-related protein LC3 A antibody
Autophagy-related ubiquitin-like modifier LC3 A antibody
LC3 antibody
LC3A antibody
MAP1 light chain 3 like protein 1 antibody
MAP1 light chain 3-like protein 1 antibody
MAP1A/1B light chain 3 A antibody
MAP1A/MAP1B LC3 A antibody
MAP1A/MAP1B light chain 3 A antibody
MAP1ALC3 antibody
MAP1BLC3 antibody
Map1lc3a antibody
Microtubule associated proteins 1A/1B light chain 3 antibody
Microtubule-associated protein 1 light chain 3 alpha antibody
Microtubule-associated proteins 1A and 1B, light chain 3 antibody
Microtubule-associated proteins 1A/1B light chain 3A antibody
MLP3A_HUMAN antibody
数据库入口号Swiss-Prot#:Q9H492
Uniprot
Q9H492
计算分子量14 kDa
实际分子量16 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.
ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MAP1LC3A in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MAP1LC3A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: MAP1LC3A Rabbit mAb at 1/1k dilutionLane 1 : K562 whole cell lysates Lane 2 : U-87 MG whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 14 kDa Observed band size: 16 kDaExposure time: 7 seconds
All lanes: MAP1LC3A Rabbit mAb at 1/1k dilutionLane 1 : Mouse brain lysates Lane 2 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 14 kDa Observed band size: 16 kDaExposure time: 6 seconds
All lanes:MAP1LC3A Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 : MAP1LC3A knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubule-associated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance micro-tubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs.
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