产品详情
产品名称Chk1 Rabbit mAb
克隆号ST57-09
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名C85740 antibody Cell cycle checkpoint kinase antibody Checkpoint , S. pombe, homolog of, 1 antibody Checkpoint kinase 1 antibody Checkpoint kinase 1 homolog (S. pombe) antibody CHEK 1 antibody Chek1 antibody Chk 1 antibody Chk1 antibody CHK1 checkpoint homolog (S. pombe) antibody CHK1_HUMAN antibody EC 2.7.11.1 antibody rad27 antibody Serine/threonine protein kinase Chk1 antibody Serine/threonine-protein kinase CHK1 antibody STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae) antibody
数据库入口号Swiss-Prot#:O14757
Uniprot
O14757
计算分子量54 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Chk1 on different lysates using anti-Chk1 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-3M
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Chk1 antibody. Counter stained with hematoxylin.
ICC staining Chk1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Chk1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Chk1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Chk1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
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