产品详情
产品名称p73 Rabbit mAb
克隆号ST05-76
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Hu, Ms，Rt
免疫原描述recombinant protein
标记Unconjugated
别名p53 like transcription factor antibody p53 related protein antibody p53-like transcription factor antibody p53-related protein antibody p73 antibody P73_HUMAN antibody TP73 antibody Tumor protein p73 antibody
数据库入口号Swiss-Prot#:O15350
Uniprot
O15350
计算分子量70/60 kDa(12 isoforms)
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of p73 on Hela cell lysates using anti-p73 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-p73 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-p73 antibody. Counter stained with hematoxylin.
ICC staining p73 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. p53 shares considerable sequence similarity with p73, a gene that maps to a region in chromosome 1 that is frequently deleted in neuroblastomas. However, p73 does not appear to be activated by DNA damaging agents. The p73 isoform p73α inhibits drug-induced apoptosis in small cell lung carcinoma cells, while the p73 isoform p73β promotes it. p73α also prevents Bax activation, mitochondrial dysfunction, caspase activation and is able to reduce apoptosis induced by the BH3-only protein PUMA (p53 upregulated modulator of apoptosis). There is an equilibrium between p73α and p73β, demonstrated by the fact that p73α inhibits the pro-apoptotic effect of p73β.
Yes
