产品详情
产品名称Chk2 Rabbit mAb
克隆号SC604
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CDS 1 antibody Cds1 antibody Cds1 homolog antibody Checkpoint kinase 2 antibody Checkpoint like protein CHK2 antibody CHEK 2 antibody Chek2 antibody Chk 2 antibody CHK2 checkpoint homolog (S. pombe) antibody CHK2 checkpoint homolog antibody CHK2_HUMAN antibody hCds1 antibody HuCds 1 antibody LFS 2 antibody LFS2 antibody PP1425 antibody RAD 53 antibody RAD53 antibody Rad53 homolog antibody Serine/threonine protein kinase Chk2 antibody Serine/threonine-protein kinase Chk2 antibody
数据库入口号Swiss-Prot#:O96017
Uniprot
O96017
计算分子量61 kDa
实际分子量61 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Chk2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Chk2 antibody. Counter stained with hematoxylin.
ICC staining Chk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Chk2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Chk2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: Chk2 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : 293T whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 61 kDa Observed band size: 61 kDaExposure time: 3 seconds
All lanes :Chk2 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type A549 cell lysateLane 2 : Chk2 knockdown A549 cell lysateLysates/proteins at 20 µg per lane.
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.
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