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Sonic Hedgehog Protein/SHH(C-Product) Rabbit mAb#48975

是否有货: Yes
产品详情

产品名称Sonic Hedgehog Protein/SHH(C-Product) Rabbit mAb

克隆号SC05-28

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

纯化ProA affinity purified

应用WB, ICC/IF, IHC, FC

种属反应性Human

免疫原描述recombinant protein

标记Unconjugated

别名HHG 1 antibody
HHG-1 antibody
HHG1 antibody
HLP 3 antibody
HLP3 antibody
Holoprosencephaly 3 antibody
HPE 3 antibody
HPE3 antibody
MCOPCB5 antibody
shh antibody
SHH_HUMAN antibody
SMMC I antibody
SMMCI antibody
Sonic Hedgehog (Drosophila) homolog antibody
sonic hedgehog homolog (Drosophila) antibody
Sonic hedgehog homolog antibody
Sonic hedgehog protein antibody
Sonic hedgehog protein C-product antibody
TPT antibody
TPTPS antibody

数据库入口号Swiss-Prot#:Q15465

Uniprot Q15465

计算分子量50 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control:
Lane 1: Hela
Lane 2: HepG2
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Sonic Hedgehog Protein antibody. Counter stained with hematoxylin.
ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Sonic Hedgehog Protein in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Sonic Hedgehog Protein in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Sonic Hedgehog Protein antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate amino- and carboxy-terminal peptides. Both proteins are secreted and appear to function in embryonic and imaginal disc patterning. Several vertebrate homologs of Drosophila hh have been identified. These include Sonic hedgehog (Shh) (alternatively designated Vhh-1), Desert hedgehog (Dhh) and Indian hedgehog (Ihh). Each contain amino-terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Shh resembles Drosophila hh in that it is processed to generate an amino-terminal secreted peptide that is retained at or near the cell surface and a carboxy-terminal glycosylated more diffusible peptide.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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