产品详情
产品名称Daxx Rabbit mAb
克隆号SC54-06
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名BING 2 antibody BING2 antibody CENP-C binding protein antibody DAP 6 antibody DAP6 antibody DAXX antibody DAXX_HUMAN antibody Death associated protein 6 antibody Death domain associated protein 6 antibody Death domain associated protein antibody Death domain-associated protein 6 antibody EAP 1 antibody EAP1 antibody ETS1 associated protein 1 antibody ETS1-associated protein 1 antibody Fas binding protein antibody Fas death domain associated protein antibody Fas death domain-associated protein antibody hDaxx antibody MGC126245 antibody MGC126246 antibody
数据库入口号Swiss-Prot#:Q9UER7
Uniprot
Q9UER7
计算分子量81 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: SW480
ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2.
如果您使用该产品48986发表了文章，请通知我们，让我们可以引用您的文献。
Yes
