Yes
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ATG9A antibody. Counter stained with hematoxylin.
ICC staining ATG9A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining ATG9A in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: ATG9A Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates Lane 2 : HepG2 whole cell lysates Lane 3 : MCF-7 whole cell lysates Lane 4 : SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDa
Exposure time: 7 seconds
All lanes: ATG9A Rabbit mAb at 1/1k dilution
Lane 1 : Mouse brain lysates Lane 2 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 94 kDa
Observed band size: 94 kDa
Exposure time: 4 seconds
All lanes:ATG9A Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : ATG9A knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
