产品详情
产品名称MyD88 Rabbit mAb
克隆号SC65-04
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名Mutant myeloid differentiation primary response 88 antibody MYD 88 antibody Myd88 antibody MYD88_HUMAN antibody MYD88D antibody Myeloid differentiation marker 88 antibody Myeloid differentiation primary response 88 antibody Myeloid differentiation primary response gene (88) antibody Myeloid differentiation primary response gene 88 antibody Myeloid differentiation primary response gene antibody Myeloid differentiation primary response protein MyD88 antibody OTTHUMP00000161718 antibody OTTHUMP00000208595 antibody OTTHUMP00000209058 antibody OTTHUMP00000209059 antibody OTTHUMP00000209060 antibody
数据库入口号Swiss-Prot#:Q99836
Uniprot
Q99836
计算分子量33 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MyD88 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MyD88 antibody. Counter stained with hematoxylin.
ICC staining MyD88 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MyD88 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MyD88 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MyD88 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Interleukin-1 (IL-1)-induced activation of the NFκB pathway is mediated through the IL-1 receptor and the subsequent phosphorylation of IL-1 receptor-associated kinase (IRAK). The myeloid differentiation protein MyD88 was originally characterized as a protein upregulated in myeloleukemic cells following IL-6-induced growth arrest and terminal differentiation. MyD88 is now known to function as an adaptor protein for the association of IRAK with the IL-1 receptor. MyD88 is functionally homologous to the adaptor protein tube in the Toll signaling pathway of Drosophilia, and both proteins are members of the Toll/IL-1R superfamily. MyD88 contains a characteristic N-terminal death domain that is essential for NFκB activation and an adjacent Toll/IL-1R homology domain (TIR domain). Collectively, these domains enable the protein-protein interactions of MyD88 with IRAK and the IL-1 receptor complex.
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