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PARK7 Rabbit mAb #49058

是否有货: Yes
产品详情

产品名称PARK7 Rabbit mAb

克隆号SN07-21

来源种属Recombinant Rabbit

克隆性 Monoclonal antibody

纯化ProA affinity purified

应用WB, ICC/IF, IHC, IP, FC

种属反应性Human;Mouse;Rat

免疫原描述recombinant protein

标记Unconjugated

别名CAP1 antibody
DJ-1 antibody
DJ1 antibody
DJ1 protein antibody
Epididymis secretory sperm binding protein Li 67p antibody
FLJ27376 antibody
FLJ34360 antibody
FLJ92274 antibody
HEL S 67p antibody
Oncogene DJ1 antibody
OTTHUMP00000001348 antibody
OTTHUMP00000001349 antibody
OTTHUMP00000001350 antibody
OTTHUMP00000001351 antibody
PARK7 antibody
PARK7_HUMAN antibody
Parkinson disease (autosomal recessive, early onset) 7 antibody
Parkinson disease protein 7 antibody
Parkinson protein 7 antibody
Protein DJ-1 antibody
SP22 antibody

数据库入口号Swiss-Prot#:Q99497

Uniprot Q99497

计算分子量20 kDa

实际分子量20 kDa

配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

保存Store at -20˚C

应用详情
WB: 1:1,000-1:2,000
IHC: 1:100-1:500
ICC: 1:100-1:500

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
ICC staining PARK7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PARK7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with PARK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: PARK7 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysates
Lane 3 : HepG2 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 3 seconds
All lanes: PARK7 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse kidney lysates
Lane 2 : Mouse brain lysates
Lane 3 : Rat kidney lysates
Lane 4 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa
Exposure time: 4 seconds
All lanes :PARK7 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : PARK7 knockdown A549 cell lysate
Lysates/proteins at 20 µg per lane.
PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies. Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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