产品详情
产品名称PARK7 Rabbit mAb
克隆号SN07-21
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP, FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名CAP1 antibody DJ-1 antibody DJ1 antibody DJ1 protein antibody Epididymis secretory sperm binding protein Li 67p antibody FLJ27376 antibody FLJ34360 antibody FLJ92274 antibody HEL S 67p antibody Oncogene DJ1 antibody OTTHUMP00000001348 antibody OTTHUMP00000001349 antibody OTTHUMP00000001350 antibody OTTHUMP00000001351 antibody PARK7 antibody PARK7_HUMAN antibody Parkinson disease (autosomal recessive, early onset) 7 antibody Parkinson disease protein 7 antibody Parkinson protein 7 antibody Protein DJ-1 antibody SP22 antibody
数据库入口号Swiss-Prot#:Q99497
Uniprot
Q99497
计算分子量20 kDa
实际分子量20 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:100-1:500
ICC: 1:100-1:500
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-PARK7 antibody. Counter stained with hematoxylin.
ICC staining PARK7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PARK7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with PARK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: PARK7 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : HepG2 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 20 kDa Observed band size: 20 kDaExposure time: 3 seconds
All lanes: PARK7 Rabbit mAb at 1/1k dilutionLane 1 : Mouse kidney lysates Lane 2 : Mouse brain lysates Lane 3 : Rat kidney lysates Lane 4 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 20 kDa Observed band size: 20 kDaExposure time: 4 seconds
All lanes :PARK7 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type A549 cell lysateLane 2 : PARK7 knockdown A549 cell lysateLysates/proteins at 20 µg per lane.
PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies. Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.
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