产品详情
产品名称MUC16 Rabbit mAb
克隆号SN0715
来源种属Recombinant Rabbit
克隆性Monoclonal
纯化ProA affinity purified
应用WB, ICC/IF, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CA 125 antibody CA-125 antibody CA125 antibody CA125 ovarian cancer antigen antibody Cancer antigen 125 antibody FLJ14303 antibody MUC 16 antibody MUC-16 antibody MUC16 antibody MUC16_HUMAN antibody Mucin 16 antibody Mucin 16 cell surface associated antibody Mucin-16 antibody Mucin16 antibody Ovarian cancer related tumor marker CA125 antibody Ovarian cancer-related tumor marker CA125 antibody Ovarian carcinoma antigen CA125 antibody
数据库入口号Swiss-Prot#:Q8WXI7
Uniprot
Q8WXI7
计算分子量2353 kDa
浓度0.333mg/ml
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
ICC: 1:100-1:500
FC: 1:50-1:100
ICC staining MUC16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MUC16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining MUC16 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with MUC16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
The mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs). Membrane-associated and secretory Mucins are high molecular weight glycoproteins of the glycocalyx (polysaccharide biofilm) that protects mucosal epithelium from particulate matter and microorganisms. Epithelial Mucins are large, secreted and cell surface glycoproteins crucial for adhesion modulation, signaling and epithelial cell protection. The number of repeats is highly polymorphic and varies among different alleles. The Mucin family consists of Mucins 1-4, Mucin 5 (AC and B), Mucins 6-8, Mucins 11-13 and Mucins 15-17. The Mucin 16 protein (also commonly referred to as CA125), encoded for by the gene MUC16, is a very high molecular weight tumor antigen consisting of three domains: a carboxy terminal domain, an extracellular domain and an amino terminal domain. Mucin 16, an ovarian cancer-associated antigen, is used as a marker to monitor the progress of epithelial ovarian cancer. It is a hydrophilic membrane-associated protein that may be involved in vitamin A functions.
Yes
