产品详情
产品名称Cyclin E1 Rabbit mAb
克隆号SD20-24
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC-P
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CCNE antibody Ccne1 antibody CCNE1_HUMAN antibody cyclin E variant ex5del antibody cyclin E variant ex7del antibody Cyclin E1 antibody Cyclin Es antibody Cyclin Et antibody CyclinE antibody G1/S specific cyclin E antibody G1/S-specific cyclin-E1 antibody
数据库入口号Swiss-Prot#:P24864
Uniprot
P24864
计算分子量48 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
ICC: 1:50-1:200
IHC-P: 1:200-1:1,000
ICC staining Cyclin E1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cyclin E1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cyclin E1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.
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