产品详情
产品名称Cdk6 Rabbit mAb
克隆号SD20-50
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名CDK 6 antibody CDK6 antibody CDK6_HUMAN antibody Cell division protein kinase 6 antibody Crk 2 antibody Crk2 antibody Cyclin dependent kinase 6 antibody Cyclin-dependent kinase 6 antibody MGC59692 antibody p40 antibody PLSTIRE antibody Serine/threonine protein kinase PLSTIRE antibody Serine/threonine-protein kinase PLSTIRE antibody STQTL11 antibody
数据库入口号Swiss-Prot#:Q00534
Uniprot
Q00534
计算分子量37 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of Cdk6 on different lysates using anti-Cdk6 antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat Lane 2: K562 Lane 3: Hela
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk6 antibody. Counter stained with hematoxylin.
ICC staining Cdk6 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Cdk6 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk6 is known to associate with cyclins D1, D2 and D3 and to be involved with the G1/S transition of the cell cycle. Multiple inhibitors of Cdk6 have been identified, including p18 and p19. These inhibitors bind to both free and complexed Cdk6, and they inhibit the activity of the cyclin D-bound Cdk6.
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