产品详情
产品名称Smad1 Rabbit mAb
克隆号SD2090
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名BSP-1 antibody
BSP1 antibody
HsMAD1 antibody
JV4-1 antibody
JV41 antibody
MAD homolog 1 antibody
MAD mothers against decapentaplegic homolog 1 antibody
Mad related protein 1 antibody
Mad-related protein 1 antibody
MADH1 antibody
MADR1 antibody
Mothers against decapentaplegic homolog 1 antibody
Mothers against DPP homolog 1 antibody
SMA- AND MAD-RELATED PROTEIN 1 antibody
SMAD 1 antibody
SMAD family member 1 antibody
SMAD mothers against DPP homolog 1 antibody
Smad1 antibody
SMAD1_HUMAN antibody
TGF beta signaling protein 1 antibody
Transforming growth factor-beta-signaling protein 1 antibody
数据库入口号Swiss-Prot#:Q15797
Uniprot
Q15797
计算分子量58 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of Smad1 on different lysates using anti-Smad1 antibody at 1/1,000 dilution. Positive control:
Lane 1: Human skeletal muscle
Lane 2: Hela
ICC staining Smad1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Smad1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with Smad1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
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