产品详情
产品名称KMT6 / EZH2 Rabbit mAb
克隆号JJ089-9
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名Enhancer of zeste 2 antibody enhancer of zeste 2 polycomb repressive complex 2 subunit antibody Enhancer of zeste homolog 2 (Drosophila) antibody Enhancer of zeste homolog 2 antibody Enhancer of zeste, Drosophila, homolog 2 antibody ENX 1 antibody Enx 1h antibody ENX-1 antibody ENX1 antibody Enx1h antibody EZH 2 antibody EZH1 antibody EZH2 antibody EZH2_HUMAN antibody EZH2b antibody Histone-lysine N-methyltransferase EZH2 antibody KMT 6 antibody KMT6 antibody KMT6A antibody Lysine N-methyltransferase 6 antibody MGC9169 antibody WVS antibody WVS2 antibody
数据库入口号Swiss-Prot#:Q15910
Uniprot
Q15910
计算分子量85 kDa
实际分子量98 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KMT6/EZH2 antibody. Counter stained with hematoxylin.
ICC staining KMT6/EZH2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining KMT6/EZH2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining KMT6/EZH2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
All lanes: KMT6 / EZH2 Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : MCF-7 whole cell lysates Lane 4 : HepG2 whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 85 kDa Observed band size: 98 kDaExposure time: 5 seconds
All lanes:KMT6 / EZH2 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type Hela cell lysateLane 2 :KMT6 / EZH2 Rabbit mAb knockdown Hela cell lysateLysates/proteins at 20 µg per lane.
In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG), which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. The gene encoding human ENX-1 transcribes a 746 amino acid polypeptide which contains a trithorax-like domain and a DNA-binding motif. ENX-1 interacts with the proto-oncogene product Vav and is thought to be involved in the proliferation of normal and malignant hematopoietic cells. By altering the regulation of target genes, ENX-1 may also contribute to certain phenotypes of Down syndrome.
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