产品详情
产品名称CDC42 Rabbit mAb
克隆号JJ086-04
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, IHC, IP, FC
种属反应性Human;Mouse;Rat
免疫原描述recombinant protein
标记Unconjugated
别名CDC42 antibody CDC42_HUMAN antibody CDC42Hs antibody Cell division control protein 42 homolog antibody Cell division cycle 42 (GTP binding protein 25kDa) antibody Cell division cycle 42 antibody dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)) antibody G25K antibody G25K GTP-binding protein antibody Growth regulating protein antibody GTP binding protein 25kDa antibody Small GTP binding protein CDC42 antibody TKS antibody
数据库入口号Swiss-Prot#:P60953
Uniprot
P60953
计算分子量21 kDa
实际分子量21 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000
IHC: 1:50-1:200
FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-CDC42 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with CDC42 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
All lanes: CDC42 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : JK whole cell lysatesLane 3 : C6 whole cell lysatesLane 4 : Mouse brain lysatesLane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 3 seconds
All lanes:CDC42 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDC42 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42.
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et al,A transcribed ultraconserved noncoding RNA, uc.285+, promotes colorectal cancer proliferation through dual targeting of CDC42 by directly binding mRNA and protein. In Transl Res on 2024 Aug by Sixian Chen, Qingyun Zhao,et al..PMID:38552953
, (2024),
PMID:
38552953
et al,A transcribed ultraconserved noncoding RNA, uc.285+, promotes colorectal cancer proliferation through dual targeting of CDC42 by directly binding mRNA and protein
, (2024),
PMID:
Yes
