产品详情
产品名称RIP Rabbit mAb
克隆号JJ092-08
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, FC
种属反应性Human
免疫原描述recombinant protein
标记Unconjugated
别名Cell death protein RIP antibody FLJ39204 antibody OTTHUMP00000039163 antibody Receptor (TNFRSF) interacting serine threonine kinase 1 antibody receptor interacting protein 1 antibody Receptor interacting protein antibody Receptor interacting protein kinase 1 antibody Receptor interacting serine threonine protein kinase 1 antibody Receptor TNFRSF interacting serine threonine kinase 1 antibody Receptor-interacting protein 1 antibody Receptor-interacting serine/threonine-protein kinase 1 antibody Rinp antibody RIP 1 antibody RIP antibody Rip-1 antibody RIP1 antibody RIPK 1 antibody Ripk1 antibody RIPK1_HUMAN antibody Serine threonine protein kinase RIP antibody Serine/threonine-protein kinase RIP antibody
数据库入口号Swiss-Prot#:Q13546
Uniprot
Q13546
计算分子量76 kDa
实际分子量76 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
FC: 1:50-1:100
Western blot analysis of RIP on Hela cells lysates using anti-RIP antibody at 1/1,000 dilution.
Flow cytometric analysis of 293 cells with RIP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
All lanes : RIP Rabbit mAb at 1/1k dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 76kDa Observed band size: 76kDa
All lanes : RIP Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : RIP knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
In contrast to growth factors which promote cell proliferation, FAS ligand (FAS-L) and the tumor necrosis factors (TNFs) rapidly induce apoptosis. Cellular response to FAS-L and TNF is mediated by structurally related receptors containing a conserved "death domain" and belonging to the TNF receptor superfamily. TRADD, FADD and RIP are FAS/TNF-R1 interacting proteins that contain a death domain homologous region (DDH). TRADD (TNF-R1-associated death domain) and FADD (FAS-associated death domain) associate with the death domains of both FAS and TNF-R1 via their DDH regions. Overexpression of TRADD leads to NFkB activation and apoptosis in the absence of TNF. Overexpression of FADD causes apoptosis, which can be blocked by the cow pox protein CrmA, suggesting that FADD lies upstream of ICE and possibly other serine proteases. The receptor interacting protein, RIP, associates with FAS exclusively via its DDH and this association is abrogated in lpr mutants. Unlike TRADD and FADD, RIP contains a putative amino terminal kinase domain.
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