Yes
Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: 293
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.
ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining eIF4EBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
All lanes : eIF4EBP1 Rabbit mAb at 1/1k dilution
Lane 1 : JK cell lysate
Lane 2 : C6 cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 13kDa Observed band size: 17kDa
All lanes : eIF4EBP1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : eIF4EBP1 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
