产品详情
产品名称Cardiac Troponin I Rabbit mAb
克隆号JF0550
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, IP
种属反应性Human;Mouse
免疫原描述recombinant protein
标记Unconjugated
别名cardiac muscle antibody Cardiac troponin I antibody cardiomyopathy, dilated 2A (autosomal recessive) antibody Cardiomyopathy, familial hypertrophic, 7, included antibody CMD1FF antibody CMD2A antibody CMH7 antibody cTnI antibody Familial hypertrophic cardiomyopathy 7 antibody MGC116817 antibody RCM1 antibody Tn1 antibody Tni antibody TNN I3 antibody TNNC 1 antibody TNNC1 antibody TNNI3 antibody TNNI3_HUMAN antibody Troponin I antibody Troponin I cardiac antibody Troponin I cardiac muscle antibody Troponin I cardiac muscle isoform antibody Troponin I type 3 cardiac antibody troponin I, cardiac 3 antibody TroponinI antibody Ttroponin I type 3 (cardiac) antibody
数据库入口号Swiss-Prot#:P19429
Uniprot
P19429
计算分子量24 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:1,000-5,000
IHC: 1:50-1:200
ICC: 1:50-1:200
Western blot analysis of Troponin I on human heart lysates using anti-Troponin I antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-Troponin I antibody. Counter stained with hematoxylin.
ICC staining Troponin I in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Troponin I in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer composed of two heavy chains (MHC) and four light chains (MLC); it interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin comprises at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites while slow/cardiac Troponin C has a single calcium binding site.
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