产品详情
产品名称PFKFB3 Rabbit mAb
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
纯化ProA affinity purified
应用WB, ICC/IF, IHC, FC, IP
种属反应性Human;Mouse;Rat
免疫原描述Recombinant protein
标记Unconjugated
别名6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
6-bisphosphatase antibody
6-P2ase 3 antibody
6-P2ASE brain/placenta-type isozyme antibody
6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme antibody
6PF 2-K/Fru 2,6 P2ase 3 antibody
6PF-2-K/Fru-2 antibody
F263_HUMAN antibody
fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase antibody
Fructose-2 antibody
Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase antibody
iPFK 2 antibody
iPFK-2 antibody
IPFK2 antibody
PFK/FBPase 3 antibody
PFK2 antibody
PFKFB3 antibody
Renal carcinoma antigen NY REN 56 antibody
Renal carcinoma antigen NY-REN-56 antibody
uPFK 2 antibody
数据库入口号Swiss-Prot#:Q16875
Uniprot
Q16875
计算分子量60 kDa
实际分子量60 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC: 1:50-1:100
IP: 1:20-1:50
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PFKFB3 antibody. Counter stained with hematoxylin.
ICC staining PFKFB3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining PFKFB3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of A431 cells with PFKFB3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
All lanes: PFKFB3 Rabbit mAb at 1/1k dilutionLane 1 : JK whole cell lysates Lane 2 : K562 whole cell lysates Lane 3 : PC12 whole cell lysates Lane 4 : Mouse spleen lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 60 kDa Observed band size: 60 kDaExposure time: 4 seconds
All lanes:PFKFB3 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HT-1080 cell lysateLane 2 :PFKFB3 Rabbit mAb knockdown HT-1080 cell lysateLysates/proteins at 20 µg per lane.
Among the enzymes playing role in glycolysis,four allosteric PFKFB enzymes 1�C4 expressed by four independent PFKFB genes, catalyze the rate-limiting phosphorylation of fructose-6-phosphate to fructose-1, 6-bisphosphate, using ATP as the energy source in the glycolysis pathway. Among these four allosteric enzymes, PFKFB3 enzyme retains the highest Kinase/Biphosphatase activity ratio and is expressed by PFKFB3 gene which has been demonstrated to be highly expressed in leukemic cells and in solid tumors. Moreover, mitogenic, hypoxic and inflammatory conditions have an inductive effect on the expression of PFKFB3. Hence upregulation of PFKFB genes specific to cancer cells compared to their normal counterparts (from the same patients) with more robust over-expression in breast and lung cancer make it a more appropriate target.
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et al,The glycolysis/HIF-1α axis defines the inflammatory role of IL-4-primed macrophages InCell Rep On2023 May 30byBuyun Dang , Qingxiang Gao et al..PMID:37149865
, (2023),
PMID:
37149865
Yes
