产品详情
产品名称Wnt2b Rabbit mAb
克隆号JB48-34
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB,IHC,ICC,IF,FC
种属反应性Human;Mouse;Rat
免疫原描述Recombinant protein
标记Unconjugated
别名Protein Wnt-13 antibody
Protein Wnt-2b antibody
Wingless type MMTV integration site family, member 13 antibody
Wingless type MMTV integration site family, member 2B antibody
WNT13 antibody
Wnt2b antibody
WNT2B_HUMAN antibody
XWNT2 antibody
XWNT2, Xenopus, homolog of antibody
数据库入口号Swiss-Prot#:Q93097
Uniprot
Q93097
计算分子量44 kDa
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC: 1:50-1:100
Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.
ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Plays a redundant role in embryonic lung development.
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et al,Modeling human ectopic pregnancies with trophoblast and vascular organoidsIn Cell Rep On2023 Jun 27byXiaoya Zhao , Zhenwu Zhang et al..PMID:37224015
, (2023),
PMID:
37224015