产品详情
产品名称KDEL Rabbit mAb
克隆号JB42-04
来源种属Recombinant Rabbit
克隆性 Monoclonal antibody
纯化ProA affinity purified
应用WB,ICC,IF,IHC,FC
种属反应性Human;Mouse;Rat
免疫原描述Recombinant protein
标记Unconjugated
别名ER lumen protein retaining receptor 1 antibody
ERD2.1 antibody
ERD21_HUMAN antibody
KDEL endoplasmic reticulum protein retention receptor 1 antibody
KDEL receptor 1 antibody
Kdelr1 antibody
Putative MAPK-activating protein PM23 antibody
数据库入口号Swiss-Prot#:P24390
Uniprot
P24390
计算分子量24/17 kDa(Predicted band size)
配方1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
保存Store at -20˚C
应用详情
WB: 1:500-1:2000
IHC: 1:100-1:400
ICC: 1:400-1:800
FC: 1:50-1:100
Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1,000 dilution.
Positive control��
Lane 1: Rat testis tissue
Lane 2: Human placenta tissue
Lane 3: Mouse testis tissue
Lane 4: 293
Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin.
ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1.
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PMID:
39826816