Yes
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CD146 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD146 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD146 antibody. Counter stained with hematoxylin.
ICC staining CD146 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD146 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HUVEC cells with CD146 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
All lanes: CD146 Rabbit mAb at 1/1k dilutionLane 1 : A375 whole cell lysates Lane 2 : HUVEC whole cell lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 72 kDa Observed band size: 120 kDaExposure time: 6 seconds
All lanes: CD146 Rabbit mAb at 1/1k dilutionLane 1 : Mouse lung lysates Lane 2 : Rat lung lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 72 kDa Observed band size: 120 kDaExposure time: 4 seconds
All lanes :CD146 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type HAP1 cell lysateLane 2 : CD146 knockdown HAP1 cell lysateLysates/proteins at 20 µg per lane.
