产品详情
产品名称TrkB (Phospho-Tyr705) Rabbit mAb
克隆号S04-6B1
来源种属Recombinant Rabbit
克隆性Monoclonal antibody
亚型IgG
纯化Affinity Purified
应用WB;ICC/IF;IHC
种属反应性Human;Mouse;Rat
免疫原描述A synthetic phosphopeptide corresponding to residues surrounding Tyr705 of human TrkB
标记Unconjugated
别名OBHD; TRKB; trk-B; EIEE58; GP145-TrkB
数据库入口号Swiss-Prot:Q16620
GeneID:4915
Uniprot
Q16620
Gene ID
4915
计算分子量92 kDa
实际分子量140 kDa
配方50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
保存Store at 4˚C short term. Aliquot and store at -20˚C long term. Avoid freeze/thaw cycles.
应用详情
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : TrkB (Phospho-Tyr705) Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysatesLane 2 : Hela treated with Lambda Protein Phosphatase for 30min whole cellLane 3 : 3T3 whole cell lysatesLane 4 : C6 whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 92 kDa Observed band size: 140 kDaExposure time: 7 seconds
Formalin-fixed;paraffin-embedded human brain tissue stained for TrkB (Phospho-Tyr705) using 52705 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence TrkB (Phospho-Tyr705) antibody (52705)
ICC/IF staining of TrkB (Phospho-Tyr705) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 52705 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit;used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
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et al,TrkB-mediated neuroprotection in female hippocampal neurons is autonomous, estrogen receptor alpha-dependent, and eliminated by testosterone: a proposed model for sex differences in neonatal hippocampal neuronal injury. In Biol Sex Differ
on 2024 Apr 2 by Vishal Chanana, Dila Zafer, et al..PMID:38566248
, (2024),
PMID:
38566248
et al,Vishal Chanana1, 2, Dila Zafer1, 2, Douglas B Kintner1, 2, Jayadevi H Chandrashekhar1, 3, Jens Eickhoff4
, (2024),
PMID:
et al,Pharmacological Co-Activation of TrkB and TrkC Receptor Signaling Ameliorates Striatal Neuropathology and Motor Deficits in Mouse Models of Huntington's Disease.
, (2023),
PMID:
37638447
Yes
