产品详情
产品名称GAPDH Rabbit mAb
来源种属Recombinant Rabbit
克隆性Monoclonal
亚型Rabbit IgG
纯化Affinity-chromatography
应用WB IHC ICC/IF IP FC
种属反应性H, M, R, Mk, Ch, Ze, Fish
特异性GAPDH Antibody detects endogenous levels of total GAPDH
免疫原描述A synthesized peptide derived from human GAPDH
标记Unconjugated
别名aging-associated gene 9 protein; G3P; G3PD; GAPD; GAPDH; glyceraldehyde 3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; MGC88685;
数据库入口号Swiss Prot:P04406
计算分子量36kDa
配方Liquid
保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.
应用详情
WB 1:5000~1:30000 IHC 1:100~1:500 ICC/IF 1:100~1:250 IP 1:50 FC 1:50
Western blot analysis of extracts from 3T3,293, Hela ,Mouse Brain and Rat Brain using GAPDH Rabbit mab #52902.
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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