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位置: 首页 > 多克隆抗体 > COL4A3 Rabbit Polyclonal Antibody

COL4A3 Rabbit Polyclonal Antibody#54194

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产品详情

产品名称COL4A3 Rabbit Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IF

种属反应性Human;Mouse;Rat

免疫原描述A synthetic peptide of human COL4A3 (NP_000082.2).

标记Unconjugated

别名COL4A3

数据库入口号Swiss Prot:Q01955
GeneID:1285

Uniprot Q01955

计算分子量135kDa/142kDa/158kDa/161kDa

实际分子量162kDa

配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.

保存Store at -20˚C. Avoid freeze / thaw cycles.

应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of 293T cells, using COL4A3 at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using COL4A3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.

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et al,Blocking ACSL6 Compromises Autophagy via FLI1‐Mediated Downregulation of COLs to Radiosensitize Lung Cancer. In Adv Sci (Weinh) on 2024 Oct by Wen Ding, Shijun Bao,et al..PMID:39206814 , (2024),
PMID: 39206814

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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