产品详情
产品名称COL4A3 Rabbit Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
亚型IgG
纯化Affinity purification
应用WB,IF
种属反应性Human;Mouse;Rat
免疫原描述A synthetic peptide of human COL4A3 (NP_000082.2).
标记Unconjugated
别名COL4A3
数据库入口号Swiss Prot:Q01955
GeneID:1285
Uniprot
Q01955
计算分子量135kDa/142kDa/158kDa/161kDa
实际分子量162kDa
配方Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存Store at -20˚C. Avoid freeze / thaw cycles.
应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot analysis of extracts of 293T cells, using COL4A3 at 1:1000 dilution.
Immunofluorescence analysis of L929 cells using COL4A3 at dilution of 1:100. Blue: DAPI for nuclear staining.
Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter.
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et al,Blocking ACSL6 Compromises Autophagy via FLI1‐Mediated Downregulation of COLs to Radiosensitize Lung Cancer. In Adv Sci (Weinh) on 2024 Oct by Wen Ding, Shijun Bao,et al..PMID:39206814
, (2024),
PMID:
39206814