Yes
All lanes: Caspase-2 Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 30,48,51 kDa
Exposure time: 4 seconds
All lanes: Caspase-2 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse heart lysates Lane 2 : Rat heart lysates Lane 3 : Rat kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 51 kDa
Observed band size: 30,48,51 kDa
Exposure time: 6 seconds
All lanes:Caspase-2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HCT116 cell lysate
Lane 2 : Caspase-2 knockdown HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for Caspase-2 using 56079 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Caspase-2 antibody (56079) ICC/IF staining of Caspase-2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56079 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
