Yes
All lanes: Cdc25C Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 60 kDa
Exposure time: 4 seconds
All lanes :Cdc25C Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Cdc25C knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Cdc25C using 56114 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Cdc25C antibody (56114) ICC/IF staining of Cdc25C in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56114 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
