Yes
All lanes: DHFR Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates Lane 2 : HepG2 whole cell lysates Lane 3 : MCF7 whole cell lysates Lane 4 : Rat kidney lysates Lane 5 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 4 seconds
All lanes: DHFR Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates Lane 2 : Mouse spleen lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 47 seconds
All lanes:DHFR Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : DHFR knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for DHFR using 56208 at 1/100 dilution in immunohistochemical analysis.
