Yes
All lanes: EEF2 Rabbit mAb at 1/1k dilution
Lane 1 : A431 whole cell lysates Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDa
Exposure time: 8 seconds
All lanes:EEF2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : EEF2 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for EEF2 using 56272 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence EEF2 antibody (56272) ICC/IF staining of EEF2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56272 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
