Yes
All lanes: CD166 Rabbit mAb at 1/1k dilutionLane 1 : MCF7 whole cell lysates Lane 2 : HepG2 whole cell lysates Lane 3 : Mouse brain lysates Lane 4 : Mouse lung lysates Lane 5 : Rat brain lysates Lysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 65 kDa Observed band size: 100 kDaExposure time: 4 seconds
All lanes :CD166 Rabbit mAb at 1/1k dilutionLane 1 : Wild-type A549 cell lysateLane 2 :CD166 knockdown A549 cell lysateLysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver tissue stained for CD166 using 48272 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CD166 using 48272 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CD166 antibody (48272) ICC/IF staining of CD166 in SKOV-3 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 48272 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
