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位置: 首页 > KO/KD抗体 > EHMT2/G9A Rabbit mAb

EHMT2/G9A Rabbit mAb#56571

是否有货: Yes
产品详情

产品名称EHMT2/G9A Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB IHC ICC/IF

种属反应性Human

特异性EHMT2/G9A Antibody detects endogenous levels of total EHMT2/G9A

免疫原描述A synthesized peptide derived from human EHMT2/G9A

标记Unconjugated

别名Bat8; Ehmt2; G9A; GAT8; NG36;

数据库入口号Uniprot:Q96KQ7

Uniprot Q96KQ7

计算分子量132 kDa

实际分子量160,170 kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

应用详情

WB:1:500~1:2000
IHC:1:50~1:200

All lanes: EHMT2 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 132 kDa
Observed band size: 160,170 kDa
Exposure time: 11 seconds
All lanes :EHMT2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : EHMT2 knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for EHMT2 using 56571 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat kidney tissue stained for EHMT2 using 56571 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence EHMT2 antibody (56571)
ICC/IF staining of EHMT2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56571 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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