Yes
All lanes: Desmoplakin Rabbit mAb at 1/1k dilution
Lane 1 : A431 whole cell lysates Lane 2 : Rat skin lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 332 kDa
Observed band size: 270 kDa
Exposure time: 6 seconds
All lanes:Desmoplakin Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Desmoplakin knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence Desmoplakin antibody (56575) ICC/IF staining of Desmoplakin in A431 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56575 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
