Yes
All lanes: ABCE1 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : HepG2 whole cell lysates Lane 4 : MCF7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 6 seconds
All lanes: ABCE1 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse liver lysates Lane 2 : Mouse spleen lysates Lane 3 : Mouse lung lysates Lane 4 : Mouse kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 7 seconds
All lanes: ABCE1 Rabbit mAb at 1/1k dilution
Lane 1 : Rat heart lysates Lane 2 : Rat liver lysates Lane 3 : Rat spleen lysates Lane 4 : Rat lung lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 8 seconds
All lanes:ABCE1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : ABCE1 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for ABCE1 using 56617 at 1/100 dilution in immunohistochemical analysis.
