Yes
All lanes: CDKN2C Rabbit mAb at 1/1k dilution
Lane 1 : MCF7 whole cell lysates Lane 2 : SH-SY5Y whole cell lysates Lane 3 : Mouse bone lysates Lane 4 : Rat bone lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 16 kDa
Exposure time: 8 seconds
All lanes:CDKN2C Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : CDKN2C knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CDKN2C using 56630 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CDKN2C antibody (56630) ICC/IF staining of CDKN2C in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56630 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
