Yes
All lanes: DLAT Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates Lane 2 : HepG2 whole cell lysates Lane 3 : MCF7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 3 seconds
All lanes: DLAT Rabbit mAb at 1/1k dilution
Lane 1 : Mouse brain lysates Lane 2 : Mouse skin lysates Lane 3 : Rat heart lysates Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 6 seconds
All lanes :DLAT Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : DLAT knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence DLAT antibody (56637) ICC/IF staining of DLAT in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56637 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
