Yes
All lanes: DGAT1 Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates Lane 2 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 48 kDa
Exposure time: 6 seconds
All lanes:DGAT1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HCT116 cell lysate
Lane 2 :DGAT1 knockdown HCT116 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for DGAT1 using 56638 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence DGAT1 antibody (56638) ICC/IF staining of DGAT1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56638 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
