Yes
All lanes: Calmegin Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 100 kDa
Exposure time: 4 seconds
All lanes:Calmegin Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Calmegin knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human testis tissue stained for Calmegin using 56682 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Calmegin antibody (56682) ICC/IF staining of Calmegin in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56682 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
