Yes
All lanes: CRKII Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates Lane 2 : Hela whole cell lysates Lane 3 : C6 whole cell lysates Lane 4 : PC12 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 38 kDa
Exposure time: 8 seconds
All lanes:CRKII Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : CRKII knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence CRKII antibody (56755) ICC/IF staining of CRKII in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56755 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
