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位置: 首页 > KO/KD抗体 > AP1G1 Rabbit mAb

AP1G1 Rabbit mAb#56877

是否有货: Yes
产品详情

产品名称AP1G1 Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB IHC

种属反应性Human;Mouse;Rat

特异性AP1G1 Antibody detects endogenous levels of total AP1G1

免疫原描述A synthesized peptide derived from human AP1G1

标记Unconjugated

别名ADTG; AP1G1; CLAPG1;

数据库入口号Uniprot:O43747

Uniprot O43747

计算分子量91kDa

实际分子量100kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

应用详情

WB:1:500~1:2000
IHC:1:50~1:200

All lanes: AP1G1 Rabbit mAb at 1/1k dilution
Lane 1 : MCF7 whole cell lysates
Lane 2 : SH-SY5Y whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 100 kDa
Exposure time: 5 seconds
All lanes: AP1G1 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse heart lysates
Lane 2 : Mouse liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 100 kDa
Exposure time: 7 seconds
All lanes: AP1G1 Rabbit mAb at 1/1k dilution
Lane 1 : Rat heart lysates
Lane 2 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 91 kDa
Observed band size: 100 kDa
Exposure time: 6 seconds
All lanes:AP1G1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : AP1G1 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for AP1G1 using 56877 at 1/100 dilution in immunohistochemical analysis.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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