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位置: 首页 > KO/KD抗体 > CC2D1A Rabbit mAb

CC2D1A Rabbit mAb#56901

是否有货: Yes
产品详情

产品名称CC2D1A Rabbit mAb

来源种属Recombinant Rabbit

克隆性Monoclonal

亚型Rabbit IgG

纯化Affinity-chromatography

应用WB IHC ICC/IF

种属反应性Human;Mouse;Rat

特异性CC2D1A Antibody detects endogenous levels of total CC2D1A

免疫原描述A synthesized peptide derived from human CC2D1A

标记Unconjugated

别名Coiled coil and C2 domain containing 1A; FREUD 1; Freud 1/Aki1; MRT3;

数据库入口号Uniprot:Q6P1N0

Uniprot Q6P1N0

计算分子量104kDa

实际分子量130kDa

配方Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

应用详情

WB:1:500~1:2000
ICC/IF:1:50~1:200
FC:1:50

All lanes: CC2D1A Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Mouse heart lysates
Lane 3 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 104 kDa
Observed band size: 130 kDa
Exposure time: 5 seconds
All lanes :CC2D1A Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CC2D1A knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CC2D1A using 56901 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CC2D1A antibody (56901)
ICC/IF staining of CC2D1A in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 56901 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

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注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk
;

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