Yes
All lanes: ACAA2 Rabbit mAb at 1/1k dilution
Lane 1 : HepG2 whole cell lysates Lane 2 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 3 seconds
All lanes: ACAA2 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse heart lysates Lane 2 : Mouse liver lysates Lane 3 : Rat heart lysates Lane 4 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
Exposure time: 6 seconds
All lanes :ACAA2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : ACAA2 knockdown A549 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human liver tissue stained for ACAA2 using 56943 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence ACAA2 antibody (56943) ICC/IF staining of ACAA2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56943 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
