Yes
All lanes: ARPC2 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates Lane 2 : JK whole cell lysates Lane 3 : HepG2 whole cell lysates Lane 4 : MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 4 seconds
All lanes: ARPC2 Rabbit mAb at 1/1k dilution
Lane 1 : Mouse liver lysates Lane 2 : Mouse lung lysates Lane 3 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 7 seconds
All lanes:ARPC2 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : ARPC2 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for ARPC2 using 56974 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence ARPC2 antibody (56974) ICC/IF staining of ARPC2 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 56974 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
