Yes
All lanes : ABRAXAS1 Rabbit mAb at 1/1k dilution Lane 1 : HepG2 whole cell lysates Lane 2 : Hela whole cell lysates Lane 3 : MCF7 whole cell lysates Lane 4 : HUVEC whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 2 seconds
All lanes : ABRAXAS1 Rabbit mAb at 1/1k dilution Lane 1 : Wild-type HT-1080 cell lysate Lane 2 : ABRAXAS1 knockdown HT-1080 cell lysate Lysates/proteins at 20 µg per lane.
Immunocytochemistry/ Immunofluorescence ABRAXAS1 antibody (57034) 57034 staining ABRAXAS1 in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 57034 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 57034 was used at a dilution of 1/100 followed by Alexa Fluor® 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.
