Yes
All lanes: Bag1 Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 33,46,50 kDa
Exposure time: 5 seconds
All lanes:Bag1 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : Bag1 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human cholangiocarcinoma tissue stained for Bag1 using 57042 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Bag1 antibody (57042) ICC/IF staining of Bag1 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 57042 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
