Yes
All lanes: AKAP 95 Rabbit mAb at 1/1k dilution
Lane 1 : HepG2 whole cell lysates Lane 2 : Mouse liver lysates Lane 3 : Rat liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 85 kDa
Exposure time: 7 seconds
All lanes:AKAP 95 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type Hela cell lysate
Lane 2 : AKAP 95 knockdown Hela cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for AKAP 95 using 57075 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence AKAP 95 antibody (57075) ICC/IF staining of AKAP 95 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 57075 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
