Yes
All lanes: CLIC4 Rabbit mAb at 1/1k dilution
Lane 1 : 293 whole cell lysates Lane 2 : RAW264.7 whole cell lysates Lane 3 : C6 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 4 seconds
All lanes :CLIC4 Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : CLIC4 knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human cholangiocarcinoma tissue stained for CLIC4 using 57118 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence CLIC4 antibody (57118) ICC/IF staining of CLIC4 in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 57118 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
